Fig. 4

Capacity and consistency across batches of bioreactor chambers (A) The pressure domain of bioreactor types relative to intracranial pressure (ICP) physiologic range; All chamber types withstood repeated pressurization cycles to 140.7 \(\:cm{H}_{2}O\) gauge pressure: significantly beyond the reported ICP domain in most hydrocephalus patients. (B) The peak amplitude of ten consecutive peaks induced by the pumps at 60 BPM pulsation rate, and 0.34 ml/min bulk flow rate. 60BPM and 0.34 ml/min were selected for their physiologic relevance to CSF production, while 50 ml/min peak amplitude waves were chosen to maximize system volatility and to “stress test” the setup and the chambers [22]. (C) The peak amplitude of 40 single inlet resin chambers manufactured in four batches (N = 10 per manufactured batch); (D) the Peak amplitude of 40 silicone chambers manufactured in 4 batches (E) The peak amplitude of five dome chambers manufactured in a single batch (F) The peak amplitude in 50 PETG chambers, manufactured in 5 batches of N = 10 per manufactured batch. Open circles indicate outliers in peak amplitude measurements