Fig. 8

Reduced amyloid burden around the pial arteries in 6-month-old Hspg2^∆3∆91;5xFAD mice. A) Timeline of the experiment showing the timing of sample collection. B) Schematic of sample collection strategy. C) Images of the pial artery stained with Col IV (green) and αSMA (red) in the mice at the ages indicated. The amyloid beta (Aβ) plaques were stained using methoxy-X04 (MX04, white). D-E) Quantification of the volume and number of MX04⁺ Aβ plaques surrounding the pial vessels shows significantly fewer Aβ plaques in the Hspg2^∆3∆91;5xFAD mice at 6 months (n = 12) than at 12 months (n = 9). F) The volume of the pial arteries shows no significant differences between the genotypes at timepoints indicated. Scale bar, 100 μm (C). The statistical test used was 2-way ANOVA followed by Sidak’s post-hoc test (D-F). *p < 0.05, **p < 0.01, ***p < 0.001. ns, non-significant. Mean ± SEM