Fig. 2

Central administration of GLP-1R modulators alters CSF flow and ICP. A Dye flow in Ex9-39-, vehicle-, or Ex4-treated rats post-i.c.v. injection with the dye intensity normalized to that obtained in the first image is plotted as a function of time, representing flow rate, n = 6. B Quantification of the dye intensity (flow rate) determined from linear regression of data in (A) over the 4 min time window. C Brain water content in rats following i.c.v. delivery of Ex4 or vehicle 24 h and 16 h prior to weighing the brains (n = 4–5). D Representative ICP traces from rats with i.c.v. delivery of Ex4, Ex9-39, or vehicle. Traces are shown before and after shift of infusion solution (marked with a grey line). E Summarized changes in the final 15 min ICP measurements (marked in (D)) compared to own baseline (shown in percentage, n = 5–6, with one outlier removed from the control group and one removed from the Ex9-39 group)). Results are shown as mean ± SEM. Statistical significance was evaluated with one-way ANOVA followed by Dunnett’s post-hoc test. *; P < 0.05, *** P < 0.001. Non significance is not shown for clarity