Fig. 3

GLP-1R expression in rat choroid plexus. A DAPI staining of choroid plexus (from the fourth ventricle) with the insert representing increased magnification of the area marked with a white box. B Representative RNAscope image illustrating mRNA expression of the Na⁺/K⁺-ATPase (Atp1a1) in the choroid plexus of the fourth ventricle with the insert representing increased magnification of the area marked with a white box. C Representative RNAscope image illustrating mRNA expression of GLP-1R (Glp1r) in the choroid plexus of the fourth ventricle with the insert representing increased magnification of the area marked with a white box. Data in A-C are from n = 3 rats with multiple images obtained in each. D Western blot of choroid plexuses (CP, from lateral and fourth ventricles) stained for GLP-1R (green) with GAPDH (red) as a loading control, n = 3. E-F Representative images of choroid plexus from the fourth ventricle in brain sections obtained from Glp1r-tdTomato reporter mice stained with DAPI (E) or imaged for the inherent fluorescence (F), with the insert representing increased magnification of the area marked with a white box, n = 3 mice with multiple images obtained in each