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Enlarged choroid plexus is linked with poorer physical function in rural older adults: a population-based study

Fluids and Barriers of the CNS volume 22, Article number: 33 (2025) Cite this article

Abstract

Background

The choroid plexus (ChP) plays an important role in producing cerebrospinal fluid (CSF) and physical dysfunction has been associated with alterations in CSF circulation. However, no population-based studies have thus far examined the association of ChP with physical function in older people.

Methods

This population-based cross-sectional study included 1217 participants (age ≥ 60 years; 57.35% women) in the MRI substudy of the Multimodal Interventions to delay Dementia and disability in rural China. ChP volume was automatically segmented using three-dimensional T1-weighted sequences. Physical function was assessed using the Short Physical Performance Battery (SPPB). Data were analyzed using general linear regression and mediation models.

Results

Controlling for demographic characteristics, cardiovascular risk factors, stroke, disproportionately enlarged subarachnoid-space hydrocephalus (DESH), and total intracranial volume, per 1-ml increase in ChP volume was associated with β-coefficient of -0.24 (95% confidence interval: -0.37 to -0.11) for SPPB summary score, with the association being stronger in females (-0.40; -0.60 to -0.20) than in males (-0.17; -0.33 to -0.01) (p for ChP volume×sex interaction = 0.028). The associations were similar across three domains of balance, chair stand, and walking speed. In addition, enlarged ChP volume was associated with increased ventricular volume and white matter hyperintensity (WMH) volume. Mediation analysis suggested that lateral ventricular volume and periventricular WMH volume significantly mediated the association of ChP volume with the SPPB summary score, with the proportion of mediation being 54.22% and 14.48%, respectively.

Conclusion

Larger ChP volume is associated with poorer physical function in older adults, especially in women. The association is largely mediated by lateral ventricular and periventricular WMH volumes.

Graphical Abstract

Background

The choroid plexus (ChP) is an endothelial-epithelial structure located in the lateral, third, and fourth ventricles that plays an important role in regulating brain homeostasis [1, 2]. The ChP is a multifunctional structure that not only produces cerebrospinal fluid (CSF) but also forms the blood-CSF barrier via tight junctions between choroidal epithelial cells. In addition, the ChP provides nutrients, a clearance system, and immune surveillance [1, 3]. In recent years, growing evidence has suggested a potential pathogenetic role of ChP and CSF formation abnormalities in neurodegenerative disorders [4,5,6,7]. Indeed, clinical-based neuroimaging studies have linked increased ChP volume with advanced stages of Alzheimer’s disease (AD) [8]. A population-based cross-sectional study of dementia-free older adults in Japan found that enlarged ChP volume was associated with poor cognitive function independent of brain parenchymal and CSF volumes [9]. In addition, a cross-sectional study of healthy adults suggested that enlarged ChP correlated with decreased ChP perfusion, increased ChP blood flow, and increased absolute CSF flow [10]. Thus, enlarged ChP may act as a compensatory mechanism to maintain sufficient CSF flow. However, chronic accumulation and malabsorption of CSF might contribute to the enlargement of ventricles. Previous studies have reported the association between ventricular dilatation and low physical function among community-dwelling older adults [11, 12]. Notably, low physical function assessed with the Short Physical Performance Battery (SPPB) is a well-established predictor for all-cause mortality [13]. In addition, several studies showed that ChP enlargement was associated with increased white matter hyperintensity (WMH) volume [14, 15]. Moreover, data from the Mayo Clinic Study of Aging (age ≥ 50 years) supported the association of greater WMH with physical dysfunction (e.g., impaired gait speed) [16]. However, the association of ChP volume with physical function and the underlying mechanisms of this association have not yet been investigated, especially in the general older population settings. This is important because understanding the possible mechanisms underlying physical impairment may help develop effective interventions to maintain physical function into older age. In addition, there are substantial sex differences in ChP volume and we previously reported that females had a lower SPPB score than males [17]. Thus, it is important to explore possible sex variations in the association of ChP volume with physical performance.

Therefore, in this population-based study, we sought to explore the association of ChP volume with physical function in rural-dwelling older adults while taking into account sex variations, and further to explore potential neuropathological mechanisms underlying the association between ChP volume and physical function.

Methods

Study design and participants

This population-based cross-sectional study used data derived from the baseline assessments of the Multimodal Interventions to delay Dementia and disability in the rural China (MIND-China), a participating project in the World-Wide FINGERS Network, a global network for dementia risk reduction and prevention [18]. The MIND-China study targeted people who were aged ≥ 60 years by the end of 2017 and living in rural communities (52 villages) in western Shandong Province. In March-September 2018, 5765 participants (74.9% of all eligible persons) underwent the baseline examination in MIND-China [19]. Participants in the MRI sub-study were recruited from 26 villages that were randomly selected from all the 52 villages in a local town. In total, 1304 participants signed the informed consent and completed the structural brain MRI scans at either Southwestern Lu Hospital (n = 1178) or Liaocheng People’ Hospital (n = 126). Of these, 87 persons were excluded due to suboptimal image quality (n = 67) or missing data on physical function (n = 20), leaving 1217 participants for the analysis involving ChP volume and physical function (see Fig. 1 for flowchart of the study participants).

Fig. 1
figure 1

Flowchart of the study participants. MIND-China, Multimodal Intervention to delay Dementia and disability in rural China; MRI, magnetic resonance imaging; SPPB, Short Physical Performance Battery

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Data collection and assessments

In March-September 2018, the trained medical staff collected data through face-to-face interviews, clinical and neurological examinations, and laboratory tests following a structured questionnaire, as previously reported [20, 21]. In brief, we collected data on demographic characteristics (e.g., age, sex, and education), lifestyle factors (e.g., smoking, alcohol intake, and physical exercise), and clinical factors (e.g., hypertension, diabetes, and hyperlipidemia). Peripheral blood samples were taken and then DNA was extracted. The APOE genotype was determined using the TaqMan single-nucleotide polymorphism (SNP) method [19] and was categorized as ε2/ε2 or ε2/ε3, ε3/ε3 (reference), and any ε4 allele. Education was categorized as no formal schooling, primary school, and middle school or above. Occupation was divided into farmers or non-farmers. Physical exercise was classified into engaging at least weekly versus less than weekly physical activity. Body mass index (BMI) was calculated as weight in kilogram divided by height in meters squared (kg/m2). Smoking and alcohol intake habits were dichotomized as current versus noncurrent smoking or alcohol intake. Hypertension was defined as self-reported physician diagnosis of hypertension or having blood pressure ≥ 140/90 mm Hg or current use of antihypertensive drugs. Diabetes mellitus was defined as self-reported history of diabetes or fasting blood glucose level ≥ 7.0 mmol/L or current use of glucose-lowering drugs or insulin injection. Hyperlipidemia was defined according to the 2016 Chinese guidelines for adults [22]: total cholesterol ≥ 6.2 mmol/L, triglycerides ≥ 2.3 mmol/L, LDL-C ≥ 4.1mmol/L, HDL-C ≤ 1.0 mmol/L or use of hypolipidemic agents. History of clinical stroke and Parkinson’s disease was ascertained according to self-reported medical history and neurological examination by a neurologist [23].

Physical function assessment

The Short Physical Performance Battery (SPPB), which consists of a standing balancing test, a 4-meter walking test, and a 5 times chair sit-to-stand test, was used to evaluate physical function [21, 22]. Each of these three physical performance tests was given a score ranging from 0 to 4. The SPPB summary score was generated by summing up scores of the three subtests (range of the summary score: 0–12), with a higher score indicating better physical performance.

Magnetic resonance imaging acquisition and processing

Structural brain images were acquired using the Philips Ingenia 3.0T MRI scanner (Philips Healthcare, Best, The Netherlands) in Southwestern Lu Hospital or the Philips Achieva 3.0T MRI scanner (Philips Healthcare, Best, the Netherlands) in Liaocheng People’ Hospital. The parameters of core MRI sequences were previously reported [24]. The 3D sT1-weighted images were processed using AccuBrain® (BrainNow Medical Technology Ltd., Shenzhen, Guangdong, China) to obtain volumes of total intracranial volumes (ICV) and lateral ventricles. The segmentation of ChP in the lateral ventricles was performed using AccuBrain® with a deep learning-based approach, as previously described [25]. Briefly, the ChP was manually labeled on 100 T1-weighted images by a trained neurologist (S.L.) and verified by a radiologist (G.T.) using ITK-SNAP software, version 4.0.1 (the Penn Image Computing and Science Laboratory, PICSL, USA) (http://www.itksnap.org). Then, the neural network was trained using these T1-weighted images, and the manually labeled ChP served as the ground truth. The network learns to recognize the unique features and patterns of ChP, allowing it to accurately segment this structure in new T1-weighted MRI scans. All automatic segmentation results were checked by two trained neurologists (S.L. and L.C.). The testing accuracy of an independent dataset (20 randomly selected subjects) achieved an average Dice coefficient of 0.82 and a volume correlation of 0.93 when comparing automatic labels with manual labels. The ChP volume was calculated automatically by scanning the automatic or manual label with the ITK-SNAP software.

WMH was defined as a hyperintense signal on T2 fluid-attenuated inversion recovery (FLAIR) images and WMH volume was acquired using AccuBrain, as previously reported [24]. In brief, T2 FLAIR images were used to calculate the signal contrast between normal brain tissue and WMH and then establish a signal threshold for WMH recognition, based on which WMHs were identified and extracted. Finally, the transformed T1-weighted brain structure mask extracted from our study samples was used to refine and localize WMHs. AccuBrain further automatically classified WMH into periventricular WMH and deep WMH following the rule of “continuity to ventricle,” while WMH < 10 mm distance from the ventricles was considered as periventricular WMH, otherwise as deep WMH [26].

Disproportionately enlarged subarachnoid-space hydrocephalus (DESH) is a neuroimaging phenotype of idiopathic normal-pressure hydrocephalus, originating from impaired CSF dynamics. We assessed the parameters of DESH related regions (i.e., the ventricular system, Sylvian fissure [SF], and subarachnoid space at high convexity and midline [SHM]) on T1-weighted images following a visual rating scale, as previously described [27]. In brief, DESH-related regions include the following three, (1) The Evans index (EI) value was the ratio of the maximum diameter of the frontal horns of the lateral ventricles to the maximum inner diameter of the skull on the transverse section. The ventricular system was classified as “not dilated” if EI ≤ 0.3 or “dilated” if EI > 0.3. Ventriculomegaly was defined as an EI > 0.3. (2) The SF was assessed in transverse and coronal sections. SF enlargement was rated as, 0 normal; 1, mildly dilated; 2, moderately dilated; and 3, severely dilated. Scores of 2 or 3 indicated enlarged SF. (3) The SHM was evaluated using transverse and coronal section images, and their tightness was rated as follows: 0, not tight; 1, moderately tight; and 2, severely tight. Scores of 1 or 2 constituted tight SHM. Participants meeting all criteria for ventriculomegaly, enlarged SF, and tight SHM were diagnosed with DESH.

Statistical analysis

We performed descriptive statistics to present characteristics of study participants by sex-specific quartiles of ChP volume, in which we reported mean (standard deviation, SD) for continuous variables and frequencies (%) for categorical variables. We used one-way analysis of variance to compare continuous variables and the chi-square test to compare categorical variables. General linear regression analyses were performed to estimate β-coefficient and 95% confidence interval (CI) of SPPB score associated with ChP volume. The ChP volume was analyzed as both a continuous variable and a categorical variable of the sex-specific quartiles. We reported the main results from 2 different models, in which different potential confounding factors were controlled for step by step: model 1 was adjusted for age, sex, education, and ICV; model 2 was additionally adjusted for physical activity, BMI, smoking, alcohol intake, hypertension, diabetes, dyslipidemia, stroke, and disproportionately enlarged subarachnoid-space hydrocephalus, and APOE genotype. We tested statistical interaction of ChP volume with sex on physical performance. When a statistical interaction was detected, further stratifying analysis was performed to assess the direction and magnitude of the interaction. Finally, we performed the mediation analysis to examine the mediation effect of lateral ventricular volume and WMH volume on the association between ChP volume and physical function, with the bootstrapping of 5000 times, while controlling for age, sex, education, ICV, physical activity, BMI, smoking, alcohol intake, hypertension, diabetes, dyslipidemia, stroke, disproportionately enlarged subarachnoid-space hydrocephalus, and APOE genotype. Several sensitivity analyses were conducted to assess the robustness of the results. IBM SPSS Statistics 25.0 for Windows (IBM Corp., Armonk, NY, USA) and R version 4.1.3 (R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/) were used for all analyses and the package bruceR in R (Bao H, 2024; version 2024.6) https://cran.r-project.org/web/packages/bruceR/index.html was used for mediation analysis.

Results

Characteristics of study participants

The mean age of the 1217 participants was 69.46 (SD = 5.5; range, 60–86) years and 57.35% were women. The mean SPPB summary score was 10.11 (SD = 2.13; range, 0–12). Compared to participants with smaller ChP volume, those with larger ChP volume were older and had larger lateral ventricular volume and WMH volume (P < 0.001). There was a trend towards an increased ChP volume being significantly associated with a decrease in SPPB summary score and in the scores of the balance, chair stand, and walk tests (all P < 0.001) (Table 1).

Table 1 Characteristics of study participants by sex-specific quartiles of choroid plexus volume
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Associations of ChP volume with physical function

Controlling for age, sex, education, and ICV, a greater ChP volume was significantly associated with lower SPPB summary score (P < 0.05) (Table 2, model 1). Adjusting for additional potential confounding factors, the association remained statistically significant (Table 2, model 2). When ChP volume was analyzed as a categorical variable (sex-specific quartiles), compared to the lowest quartile, the top quartile of ChP volume was significantly associated with a reduced SPPB summary score (P < 0.01) (Table 2, model 1); adjusting for additional potential confounding factors, the highest quartile of ChP volume remained significantly associated with the lower SPPB summary score (P < 0.01) (Table 2, model 2).

Table 2 Associations of choroid plexus volume with SPPB summary score (n = 1170)
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We detected statistical interactions of ChP volume with sex on the SPPB summary score (Pfor ChP volume×sex interactions < 0.05). Stratified analysis by sex suggested that the association of larger ChP volume with lower SPPB summary score was stronger in females than in males in the multivariable-adjusted model (Fig. 2, model 1); the associations remained significant in both females and males after additionally adjusting for additional potential confounding factors (Fig. 2, model 2).

Fig. 2
figure 2

Associations of choroid plexus volume with physical function assessed with SPPB summary score by sex. Abbreviations: ChP, choroid plexus; SPPB, Short Physical Performance Battery. Note: Model 1 was controlled for age, education, and total intracranial volume; model 2 was additionally adjusted for smoking, alcohol intake, body mass index, physical exercise, hypertension, diabetes, dyslipidemia, APOE genotype, stroke, and disproportionately enlarged subarachnoid-space hydrocephalus. *P < 0.05, **P < 0.01

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Associations of choroid plexus volume with WMH volume and ventricular volume

Controlling for age, sex, education, and ICV, a greater ChP volume was significantly associated with larger total WMH volume and periventricular WMH volume (both P < 0.01) (Table 3, model 1). Adjusting for additional potential confounding factors, the association remained statistically significant (Table 3, model 2). However, larger ChP volume was not significantly associated with deep WMH volume (P > 0.05) (Table 3, models 1 and 2). In addition, after adjusting for age, sex, education, and ICV, larger ChP volume was also significantly related to larger lateral ventricular volume and inferior lateral ventricular volume (both P < 0.01) (Table 3, model 1). Adjusting for additional potential confounding factors, the associations remained statistically significant (Table 3, model 2).

Table 3 Associations of choroid plexus volume with white matter hyperintensity volume and ventricular volume (n = 1165)
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Mediation of lateral ventricular volume and periventricular WMH volume in the association of ChP volume with physical function

We performed parallel mediation analysis to further explore the extent to which ventricular volume and WMH volume could mediate the cross-sectional association between ChP volume and physical function. Controlling for demographics, vascular risk factors, total ICV, physical exercise, APOE genotype, stroke, and disproportionately enlarged subarachnoid-space hydrocephalus, larger lateral ventricular volume and periventricular WMH volume, which were significantly associated with lower SPPB summary score (both P < 0.01), could significantly mediate 54.22% and 14.48%, respectively, of the association between ChP volume and the SPPB summary score (Fig. 3).

Fig. 3
figure 3

Mediation effects of MRI markers on the associations of choroid plexus volume with physical function. Abbreviations: ChP, choroid plexus; SPPB, Short Physical Performance Battery.Note: The model was adjusted for age, sex, education, total intracranial volume, body mass index, physical exercise, ever smoking, alcohol intake, hypertension, diabetes, dyslipidemia, APOE genotype, stroke, and disproportionately enlarged subarachnoid-space hydrocephalus. *P < 0.05, **P < 0.01

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Sensitivity analysis

The following sensitivity analyses were performed to further assess the robust association between ChP volume and physical function. First, we examined the relationship of ChP volume with scores of three SPPB subtests (e.g., the balance, chair stand, and walking speed tests). Controlling for potential confounding factors, a greater ChP volume was significantly associated with lower scores of three SPPB subtests (all P < 0.05) (Table S1, Models 1 and 2). When ChP volume was analyzed as a categorical variable (sex-specific quartiles), compared to the lowest quartile, the top quartile of ChP volume was significantly associated with a lower balance score and a reduced chair stand test score (all P < 0.01) (Table S1, Models 1 and 2). Furthermore, we repeated the analysis by excluding participants with dementia (n = 29) and Parkinson’s Disease (n = 2). The relationships of ChP volume, both as continuous and categorical variables, with SPPB summary score remained statistically significant after adjusting for potential confounding factors (Table S2, Models 1 and 2). We also repeated the analysis by further excluding 272 participants with mild cognitive impairment from the dementia-free participants. The associations of ChP volume, as a continuous variable, with SPPB summary score remained statistically significant (P = 0.007, data not shown). Finally, we repeated the analysis by excluding participants from Liaocheng People’ Hospital (n = 97). The relationships of ChP volume, both as continuous and categorical variables, with SPPB summary score were similar to results from the main analysis (Table S3, Models 1 and 2).

Discussion

The main findings from this population-based study of rural-dwelling older adults in China can be summarized as follows: (1) the larger ChP volume was associated with lower physical function; (2) the associations of larger ChP volume with worse physical function varied with sex, such that the associations were evident mainly in females; and (3) the association of enlarged ChP volume with poor physical function was largely mediated by the lateral ventricular volume and periventricular WMH.

Previously, a clinical-based study of patients with early-stage Parkinson’s disease showed that larger ChP volume was associated with motor deficits [28]. In a population-based study of dementia-free older adults in Japan, an increased ChP volume was found to be cross-sectionally correlated with a lower Mini-Mental State Examination score [9]. To the best of our knowledge, our study is the first population-based study that explores the association of ChP volume with physical function among older adults. We found that a greater ChP volume was associated with poorer physical function, including impaired balance, chair stand, and gait speed. We further explored the potential mechanisms linking enlarged ChP volume with physical dysfunction. Firstly, we explored whether the association of enlarged ChP volume with poor physical function was largely dependent on ventricular volume. This is important because pathophysiologically, enlarged ChP and lateral ventricles are closely related [10, 29], and several population-based studies have reported the association between ventricular volume and physical function in older adults. For instance, the cross-sectional data from the US Cardiovascular Health Study showed that larger ventricular volume was associated with balance disorders [30]. Furthermore, the cross-sectional data from the Icelandic Age, Gene/Environment Susceptibility-Reykjavik Study suggested that ventricular dilation was associated with gait impairment [31]. Similarly, the Mayo Clinic Study of Aging suggested that ventricular enlargement was strongly associated with slower gait speed [12]. Therefore, it is plausible to hypothesize that the association of enlarged ChP with physical impairment could be, at least partly, attributable to enlarged lateral ventricle. Then, we explored the role of WMH volume in the association of enlarged ChP volume with poor physical function. Previously, population-based studies have shown that enlarged ChP volume are closely related to larger WMH volume [14, 15]. Data from the Alzheimer’s Disease Neuroimaging Initiative also showed that a greater WMH burden was associated with enlarged ChP volume in older adults [32]. Moreover, several studies have linked increased WMH volume with physical dysfunction in older adults [33,34,35,36,37]. For example, the cross-sectional data from the Guangdong SVD study in China showed that WMH is associated with motor dysfunction [34]. In support of this hypothesis, our population-based study indeed revealed for the first time that lateral ventricular volume and periventricular WMH could partly mediate the cross-sectional association between enlarged ChP volume and low physical function. The mechanisms underlying the ChP volume-physical function association among older adults are not fully understood. Previous studies have shown that neuroinflammation is involved in abnormal deposition of lipofuscin and amyloid in ChP epithelial cells, which in turn could result in epithelial edema and ChP enlargement [38, 39]. In addition, pro-inflammatory cytokines might promote the differentiation or proliferation of nonimmune cells, and further lead to ChP enlargement [40]. Enlarged ventricles may impede frontal projections and midbrain, the vital anatomical structures for regulating balance and gait, thus leading to physical dysfunction (e.g., impaired balance and gait speed) [41,42,43]. Moreover, neuroinflammation is known to play a crucial role in the pathogenesis of cerebral small vessel disease (CSVD) [44]. Gait impairment associated with CSVD is likely caused by interrupted frontal cortical–subcortical circuits and decreased connectivity of cerebral white matter tracts in older adults [45].

We detected the sex-varying association between larger ChP volume and lower physical performance, such that a larger ChP volume showed a stronger association with lower physical performance in females than in males. The biological mechanisms underlying the sex-varying association between the ChP volume and physical function are unknown. In the MIND-China cohort, the prevalence of obesity, hypertension, diabetes, and dyslipidemia were higher in females than males [19, 46, 47]. Previous studies have correlated cardiometabolic risk factors (e.g., hypertension and obesity) with enlarged ChP volume [8, 48]. However, our study indicated that the sex differences in the association of larger ChP volume with poorer physical function was present independent of major cardiometabolic risk factors. Further research may help elucidate neuropathological mechanisms underlying the sex differences in their association.

This large-scale population-based MRI study engaged older adults who were living in rural communities in China, a sociodemographic group that has been underrepresented in research of aging and physical function. However, our study also has limitations. First, a temporal relationship of ChP volume with poor physical performance cannot be determined owing to the nature of cross-sectional design. Second, we did not use the gold standard manual annotation for ChP segmentation due to time-consuming and cumbersome in a large sample, instead, we used the deep learning models to automatically segment ChP volume, which is considered a simple and highly reliable solution [25]. Third, the study sample was drawn from a single rural area of western Shandong Province where a considerable proportion of participants had received no or very limited school education. This should be kept in mind when generalizing the results of our study to other populations.

In conclusion, this population-based cross-sectional study provides evidence in favor of the hypothesis that enlarged ChP volume is associated with poorer physical function in rural older adults. Of note, we revealed that the lateral ventricular volume and WMH volume could largely mediate the ChP volume-physical function association, suggesting that ventricular enlargement due to ChP malfunction may be a biological mechanism or pathway underlying physical dysfunction. Future prospective follow-up studies are warranted to further explore the potential causal relationship of ChP volume with subsequent consequences of physical function as well as the mechanisms underlying their association in older adults.

Data availability

No datasets were generated or analysed during the current study.

Abbreviations

ChP:

Choroid plexus

CSF:

Cerebrospinal fluid

SPPB:

Short Physical Performance Battery

MIND-China:

The Multimodal Interventions to delay Dementia and disability in rural China

AD:

Alzheimer’s disease

MCI:

Mild cognitive impairment

BMI:

Body mass index

ICV:

Intracranial volumes

DESH:

Disproportionately enlarged subarachnoid-space hydrocephalus

CI:

Confidence interval

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Acknowledgements

We would like to thank the medical staff at the Yanlou Town Hospital, and the MIND-China Research group at the Shandong Provincial Hospital for their invaluable contributions to data collection and management.

Funding

The MIND-China Project was financially supported by the STI2030-Major Project (grant no.: 2021ZD0201808 and 2022ZD0211600) and by additional grants from the National Key R&D Program of China (grant no.: 2017YFC1310100), the National Nature Science Foundation of China (grants no.: 82171175, 82011530139, and 82001120), the Academic Promotion Program of Shandong First Medical University (grants no.: 2019QL020 and 2020RC009), and the Taishan Scholar Program of Shandong Province (grants no.: ts20190977 and Tsqn201909182). This work was supported by additional grants from the Nature Science Foundation of Shandong Province (grant no.: ZR2024MH225), and Integrated Traditional Chinese and Western Medicine Program in Shandong Province (YXH2019ZXY008). D Ferreira received funding from the Swedish Research Council (Vetenskapsrådet, grant no.: 2022-00916), the Center for Innovative Medicine (CIMED, grants no.: 20200505 and FoUI-988826), the regional agreement on medical training and clinical research of Stockholm Region (ALF Medicine, grants no.: FoUI-962240 and FoUI-987534), the Swedish Brain Foundation (Hjärnfonden, grants no.: FO2023-0261, FO2022-0175, and FO2021-0131), the Swedish Alzheimer Foundation (Alzheimerfonden, grants no.: AF-968032, AF-980580, and AF-994058), the Swedish Dementia Foundation (Demensfonden), the Gamla Tjänarinnor Foundation, the Gun och Bertil Stohnes Foundation, the Funding for Research from Karolinska Institutet, Neurofonden, and the Foundation for Geriatric Diseases at Karolinska Institutet, as well as contributions from private bequests. C Qiu received grants from the Swedish Research Council (grants no.: 2017-05819 and 2020-01574) for the Sino-Sweden Joint Research Projects and the Swedish Foundation for International Cooperation in Research and Higher Education (STINT) (grant no.: CH2019-8320) for the Joint China-Sweden Mobility program, Stockholm, Sweden.

Author information

Authors and Affiliations

  1. Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, P.R. China

    Qianqian Xie, Ziwei Chen, Jiafeng Wang, Huisi Zhang, Yan Wang, Xiaoyu Wang, Chunyan Li, Yongxiang Wang, Lin Cong, Lin Song, Yifeng Du & Chengxuan Qiu

  2. Department of Neurology, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, P.R. China

    Yongxiang Wang, Lin Cong, Lin Song & Yifeng Du

  3. Shandong Provincial Clinical Research Center for Neurological Diseases, Jinan, Shandong, P.R. China

    Yongxiang Wang, Lin Cong, Lin Song & Yifeng Du

  4. Institute of Brain Science and Brain-Inspired Research, Shandong First Medical University, Shandong Academy of Medical Sciences, Jinan, Shandong, P.R. China

    Yongxiang Wang, Yifeng Du & Chengxuan Qiu

  5. Aging Research Center, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet-Stockholm University, Stockholm, Sweden

    Yongxiang Wang, Anna-Karin Welmer & Chengxuan Qiu

  6. Division of Clinical Geriatrics, Center for Alzheimer Research, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm, Sweden

    Daniel Ferreira

  7. Facultad de Ciencias de la Salud, Universidad Fernando Pessoa Canarias, Las Palmas, Spain

    Daniel Ferreira

  8. Division of Physiotherapy, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm, Sweden

    Anna-Karin Welmer

  9. Medical Unit Medical Psychology, Women´s Health and Allied Health Professionals Theme, Karolinska University Hospital, Stockholm, Sweden

    Anna-Karin Welmer

Authors
  1. Qianqian Xie
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  2. Ziwei Chen
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  3. Jiafeng Wang
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  4. Huisi Zhang
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  5. Yan Wang
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  6. Xiaoyu Wang
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  7. Chunyan Li
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  8. Yongxiang Wang
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  9. Lin Cong
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  10. Daniel Ferreira
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  11. Anna-Karin Welmer
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  12. Lin Song
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  13. Yifeng Du
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  14. Chengxuan Qiu
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Contributions

QX: Conceptualization; Data curation; Formal analysis; Investigation; Methodology; Project administration; Resources; Software; Validation; Visualization; Roles/Writing - original draft and Writing - review & editing. ZC: Data curation; Investigation and Resources; JW: Data curation; Formal analysis; Investigation and Resources. HZ: Data curation; Formal analysis; Investigation and Resources. YW: Data curation; Investigation and Resources; XW: Formal analysis. CL: Data curation and Resources. YW: Data curation; Investigation and Resources. LC: Data curation; Investigation and Resources. DF: Writing - review & editing. AKW: Writing - review & editing. LS: Conceptualization; Data curation; Investigation; Methodology; Project administration; Resources; Supervision and Writing - review & editing. YD: Conceptualization; Data curation; Funding acquisition; Investigation; Supervision and Project administration. CQ: Conceptualization; Data curation; Funding acquisition; Investigation; Project administration; Resources; Supervision and Writing - review & editing. All authors approved the final manuscript.

Corresponding authors

Correspondence to Lin Song or Yifeng Du.

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Ethics approval and consent to participate

The MIND-China protocol was approved by the Ethics Committee at Shandong Provincial Hospital Affiliated to Shandong First Medical University in Jinan. Written informed consent was obtained from all the participants or from a proxy (a guardian or a family member) if participants were not able to give the consent. The MIND-China study was registered in the Chinese Clinical Trial Registry (registration No.: ChiCTR1800017758).

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All authors gave their consent for publication.

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The authors declare no competing interests.

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Xie, Q., Chen, Z., Wang, J. et al. Enlarged choroid plexus is linked with poorer physical function in rural older adults: a population-based study. Fluids Barriers CNS 22, 33 (2025). https://doiorg.publicaciones.saludcastillayleon.es/10.1186/s12987-025-00642-z

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Keywords

Fluids and Barriers of the CNS

ISSN: 2045-8118